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qPCR normalization

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Old 07-03-2008, 07:58 PM
Pipette Filler
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Default qPCR normalization
Hi, I am doing qPCR (copy number) and I am analizing using absolute quantification (dilution curve). Do I have to use a housekeeping gene to normalize the results? Thanks.
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  #2 (permalink)  
Old 07-11-2008, 03:33 PM
Pipette Filler
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Default Re: qPCR normalization
Quote:
Originally Posted by danush View Post
Hi, I am doing qPCR (copy number) and I am analizing using absolute quantification (dilution curve). Do I have to use a housekeeping gene to normalize the results? Thanks.
Hi

yeah sure , rather i would recommend to use a pool of housekeeping genes as using only one or two genes might lead to false results. I hope it helps
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