rtpcr

[nadia naeem]

while doing rt pcr when i am using 20pM conc. of primer
i did not get band in -ve ctrl,
i got band in +ve ctrl
and light band in test.
good results
but.....
in order to increase the darkness of band in test i increase the conc of primer to 40pM and 100pM now i am getting more than two bands in ctrl as well as in test

can any one tell me whats the reason behind it

[Mehdi]

Originally Posted by nadia naeem

while doing rt pcr when i am using 20pM conc. of primer
i did not get band in -ve ctrl,
i got band in +ve ctrl
and light band in test.
good results
but.....
in order to increase the darkness of band in test i increase the conc of primer to 40pM and 100pM now i am getting more than two bands in ctrl as well as in test

can any one tell me whats the reason behind it

Hi ,

I was just wondering if we could clone a qPCR product. Is syber green going to create any problem?