Aliquotting primers...together?

[jcosborn06]

I will be running qPCR using identical, unchanging quantities of forward and reverse primers over a period of time. I'd like to make primer aliquots with the FW and REV primers in the same tube, as this would simplify my qPCR setup. Is there any reason I should keep the FW and REV primers separate for storage purposes?

I thought for a moment that this would be conducive to primer-dimer formation, and then recalled that everything should be denatured anyway in the beginning of my qPCR. But hearing someone agree with me would be nice.

[amberpdidit]

Nope, you'll be fine. In fact this is common practice to have a primer mix. Just remember to make the final concentration of each what you want it to be.