| | Re: Standard Curve
Are you diluting the RNA? Maybe your quality isn't what you think. I would check RNA quality (not just quantity, if you have access to a bioanalyzer that'd be good)
The other thing I would consider is doing smaller dilutions, maybe 2 fold. You may just be in a wierd range for detection.
Good luck, this stuff can be so frustrating.
Wait, I just saw that your NTC is coming up. That is a big problem. I think you have some contaminant somewhere. Start with your water and other buffers and if you are seeing something in NTC than you need to fix that.
Are you doing 1-step or 2-step? TaqMan or SYBR?