|Register||Search||Today's Posts||Mark Forums Read|
|Real-Time PCR and Quantitative PCR Forum Real-Time PCR and Quantitative PCR Forum|
| ||LinkBack||Thread Tools||Display Modes|
Cell lysis buffer compatible with reverse transcription and real time RT-PCR
Does any know of a cell lysis buffer that is compatible with downstream real time RT-PCR analysis? That is, a buffer that lyse the cells and which can be used directly for reverse transcription so no RNA isolation is required. If anyone would be willing to share such a buffer recipe or has come across a relevant paper/reference I would very much appreciate it.
I am aware that such a lysis buffer is commercially available from, forexample, Ambion (Cells-to-cDNA™ II Cell Lysis Buffer) but it is somewhat expensive.
|buffer , cell , compatible , lysis , real , reverse , rtpcr , time , transcription|
|Thread||Thread Starter||Forum||Replies||Last Post|
|Human Cytome Project - Update 24 Jan. 2005||Peter Van Osta||Cell Biology and Cell Culture||1||08-01-2010 02:18 PM|
|Human Cytome Project - Update 6 Jan. 2005||Peter Van Osta||Cell Biology and Cell Culture||0||01-06-2005 10:18 AM|