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tr0lle 02-20-2008 01:06 PM

Cell lysis buffer compatible with reverse transcription and real time RT-PCR
 
Hey

Does any know of a cell lysis buffer that is compatible with downstream real time RT-PCR analysis? That is, a buffer that lyse the cells and which can be used directly for reverse transcription so no RNA isolation is required. If anyone would be willing to share such a buffer recipe or has come across a relevant paper/reference I would very much appreciate it.

I am aware that such a lysis buffer is commercially available from, forexample, Ambion (Cells-to-cDNA™ II Cell Lysis Buffer) but it is somewhat expensive.


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