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Real time PCR - neophyte ..

Real time PCR - neophyte .. - Real-Time PCR and Quantitative PCR Forum

Real time PCR - neophyte .. - Real-Time PCR and Quantitative PCR Forum


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Old 02-08-2008, 09:15 PM
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Question Real time PCR - neophyte ..



Hey everyone,
Dumb thread - maybe dumb question. Ours was a "conventional" PCR lab and now we have purchased a real time machine. I'm the only one "deputed" to work on it. The protocols for real time-PCR is clear. But could someone tell me how to interpret the data afterwards. I've sifted through a lot of literature on the net, but am still confused. Could someone please explain to me - stepwise how to do the data interpretation part.
Would appreciate any help.
"the confused one"
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Old 02-08-2008, 09:41 PM
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Default Re: Real time PCR - neophyte ..

Yeah sure. Some info first though.
Just mention what dye for RT-PCR, SYBR? Taqman?
The number of PCR cycles and what you are using for Negative and Positive control (should be a dilution series).
DNA or RNA? General size of PCR products.
Extraction technique and input DNA concentration for samples.
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