Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > PCR - Polymerase Chain Reaction Forum > Real-Time PCR and Quantitative PCR Forum
Register Search Today's Posts Mark Forums Read

Real-Time PCR and Quantitative PCR Forum Real-Time PCR and Quantitative PCR Forum


Help!!

Help!! - Real-Time PCR and Quantitative PCR Forum

Help!! - Real-Time PCR and Quantitative PCR Forum


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 11-22-2007, 10:41 AM
Pipette Filler
Points: 864, Level: 16 Points: 864, Level: 16 Points: 864, Level: 16
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2007
Posts: 0
Thanks: 0
Thanked 0 Times in 0 Posts
Default Standard Curve for Another Plate Real Time PCR



Hello! Do you know how I can use one standard curve for another plate?
I think that I have to use the equation y=mx+b.. but I can´t understand how!
My values are:
slope= - 3.434819
intercept= 47.734550
R2= 0.993460

Tanks

Last edited by admin; 11-22-2007 at 03:18 PM.
Reply With Quote
  #2  
Old 11-26-2007, 05:12 PM
danfive's Avatar
Nobel Laureate
Points: 8,612, Level: 64 Points: 8,612, Level: 64 Points: 8,612, Level: 64
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2007
Location: Houston TX
Posts: 969
Thanks: 12
Thanked 184 Times in 154 Posts
Default Re: Help!!

It is best to avoid doing this. The only exception is if you have a very standardized protocol and use plasmid or viral vector for standard curve, (which would be very stable across runs). Only if you have that kind of documented, reference standard curve should you try it.
Reply With Quote
Reply


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump


All times are GMT. The time now is 09:30 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11087 seconds with 15 queries