Primer dilution problem in RAPD PCR

[richadeo]

Hi guys,
I am working on genetic diversity of plant species using RAPD technique. I have ordered random primers in lyophilized form. I need to dilute them before using in pCR mix. How to make dilution for making working solution?
Can anybody please help me out.

[megha]

hello,
i am also working on the same,,,,
primer must be provided with further necessary informations for dilution,,
otherwise you should visit on website for operon primer dilution..

megha
INDIA

[Priyanka]

Hi
Its very simple... There would be given necesary specifications on the vial indicating the micromolar /pico-molar concentration of the primer just use standard formulas.. (the dealer would have given you a written handout for that also use it...(they sometimes mention how mush water shud you take for what dilution...)) Just chill and calculate you`d be fine...
Cheers!!
Priyanka

[chovek69]

After redisolution of the lyophilised primer you should always check the conc. spectrophotometrically. Don't believe to the signs. I used to have alot of headaches in the past when blindly believing on the manufacterers' numbers.