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| Hi, I'm fairly new to qPCR and would love to find a good tutorial on primer design. I'm currently using Invitrogen Vector NTI software, but I've tried other tools and they all see to spit out a bunch of likely looking options. Specifically, how do I decide on the most important design considerations? Should I be especially concerned about Tm or are primer dimers and hairpins a bigger worry? I've received lots of helpful advice from around the lab, but it seems like a mash of rules of thumb (avoid 4 A in a row). If anyone knows of a good site, or has a list of rules written down, that would certainly help me. Thanks, mike |
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