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05-22-2007, 07:49 AM
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 Real-time PCR instrument Hello! I'm living in France and, in my lab, we are looking for a new real-time PCR instrument using 96-wells plates. At this time, we have to decide between: - Bio-Rad Chromo4 - Roche Light-Cycler480 - Eppendorf Mastercycler Realplex4 Do you use them ? Have you got some problem (technical, software, manipulations?) All your comments are welcome ! Thanks !        |
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05-22-2007, 01:12 PM
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 Re: Real-time PCR instrument Hi We have a Bio Rad mini opticon of 48 wells. It has been used in the lab for a year or so...recently we had some probelms with the lid and with the machine stopping at a certain point in a melting curve...we had prompt response from the Bio Rad people...we are now waiting for it to be repaired and meanwhile they have sent another machine to replace the other one. I think that if you're thinking of using the Universal Probe Library from Roche, I would go for the Roche machine. However, Bio rad also supports the taqman technology in most of the models good luck Anna |
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07-23-2007, 04:08 PM
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| Re: Real-time PCR instrument They should be fine but Applied Biosystems and BioRad are the best in terms of software and support. I've used both systems for real-time pcr Applied bio platform was great for Taqman probes, and BioRad was good for SYBR and taqman. |
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