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Real-time PCR instrument

Real-time PCR instrument - Real-Time PCR and Quantitative PCR Forum

Real-time PCR instrument - Real-Time PCR and Quantitative PCR Forum


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  #1  
Old 05-22-2007, 07:49 AM
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Exclamation Real-time PCR instrument



Hello! I'm living in France and, in my lab, we are looking for a new real-time PCR instrument using 96-wells plates.
At this time, we have to decide between:
- Bio-Rad Chromo4
- Roche Light-Cycler480
- Eppendorf Mastercycler Realplex4

Do you use them ? Have you got some problem (technical, software, manipulations?)
All your comments are welcome !
Thanks !
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Old 05-22-2007, 01:12 PM
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Default Re: Real-time PCR instrument

Hi
We have a Bio Rad mini opticon of 48 wells. It has been used in the lab for a year or so...recently we had some probelms with the lid and with the machine stopping at a certain point in a melting curve...we had prompt response from the Bio Rad people...we are now waiting for it to be repaired and meanwhile they have sent another machine to replace the other one.

I think that if you're thinking of using the Universal Probe Library from Roche, I would go for the Roche machine. However, Bio rad also supports the taqman technology in most of the models

good luck

Anna
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Old 07-23-2007, 04:08 PM
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Default Re: Real-time PCR instrument

They should be fine but Applied Biosystems and BioRad are the best in terms of software and support. I've used both systems for real-time pcr Applied bio platform was great for Taqman probes, and BioRad was good for SYBR and taqman.
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Old 07-30-2007, 06:39 AM
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Default Re: Real-time PCR instrument

Thaks for your answers !
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