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| HI which type of RT-PCR is better for experimentation if we consider economically and also efficiency of performance. 1- With SYBR green dye 2-FRET-RT-PCR what are the advantages and disadvantages of these PCR. Waiting for answer aftab |
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| I would also choose SYBR green dye. The advantages of the SYBR Green dye chemistry are as follows: • It can be used to monitor the amplification of any double-stranded DNA sequence. • No probe is required, which reduces assay setup and running costs. Disadvantage of SYBR Green I Dye The primary disadvantage of the SYBR Green I dye chemistry is that it may generate false positive signals; i.e., because the SYBR Green dye binds to any double-stranded DNA, it can also bind to nonspecific double-stranded DNA sequences. |
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