| | Re: expression levels of different genes within the same sample
thanks for your time to write this detailed instructions!
unfortunatelly, I have a feeling that I did not explain well my problem/question.
so, usually you want to compare expression levels of desired gene(s) between two sets of samples (control and treated, cell/tissue-1 and cell/tissue-2, or so on...). it is clear to me how to do that.
what I do not know how to do is to compare expression levels of gene A and gene B (and C, D, E...) within the _same sample_, so to be able to answer if A is expressed at higher levels than B (and so on...). absolute quantification would give me desired answer (i.e. x moleculs of A, y moleculs of B), but I am not sure how/if I could obtain this answer if not using standard curve with apsolute concentrations (from plasmid, for example).
so far, I came to conclussion that this would not be possible to do (or would be too demanding) with SyberGreen technology since ct-s depend on the length of Pcr products, among other things. maybe with TaqMan it would be feasable, but I did not encounter relevant papers or software for that kind of analysis.
hope I made myself more clear this time.
thank you anyway,