How to wash cells in 96 well plate?

[eunis]

I lost most of my primary endothelial cells after PBS wash and aspiration. I coated the wells with fibronectin, which is recommended by the vender. Every time I tilted the plate at an angle and vacuumed all the solution with a sterile pipette on the side of the well. I'm sure that the ECs should attach well to the bottom. I need 20000-100000 cells at the end to measure apoptosis. That make me so frustrated! What technique or specific care should I take?

[Anuska]

I have the same problem. Every time I wash my T47D cells, I wash away most of the cells. They seem not attaching very well. I tryes to vacum with a pump and with the multichannel pippete....and always depend on how happy my cells are attached to the bottom of the plate..

[mcphee357]

We are having the same problem here with 3 adherent cell lines. Iv read that using 24-well plates are the way forwards. I havent tested this out yet but will be over the next few days. What assay are you doing?