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Alkaline Phosphotase Treatment

Alkaline Phosphotase Treatment - Protocols and Methods Forum

Alkaline Phosphotase Treatment - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 05-29-2013, 07:14 PM
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Default Alkaline Phosphotase Treatment



Hi,

I need help foe below issue.

I see two bands for my protein of interest on the western blot. I want to remove (or decrease intensity) the phosphorylated band by alkaline phosphotase treatment ( or any other idea?). I could not find a protocol for alkaline phosphotase treatment? I would really appreciate any help.

By the way, I work with plant tissue

Thanks

Gokhan
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alkaline , dephosphorylation , phosphates , phosphotase , protein identification , treatment


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