I have a question about animal-derived laboratory reagents and products. I’m working in a strictly in-vitro lab that focuses mainly on western blots and flow cytometry. I was wondering the cost to animal life associated with these reagents. These include: FBS (fetal bovine serum) & BSA (bovine serum albumen):
I figured these products are likely a byproduct of the slaughterhouse industry and therefore the animals are being slaughtered more for the meat than to get the blood to make these products. Am I right in thinking this? Primary & secondary antibodies (raised in mouse, rabbit, goat, etc.):
From what I understand there are three main types with differing degrees of cost to animal life
• In vitro Monoclonal antibodies:
Once the hybridoma is created they can be grown indefinitely in vitro so there is minimal cost to animal life. Am I right in thinking this?
• In vivo (ascites) monoclonal antibodies:
Does an animal have to die every time I place a new order for an antibody?
• Polyclonal antibodies:
Again, does an animal have to die every time I place a new order for an antibody? Sheep’s blood agar:
Unlike the bovine products, I wouldn’t think sheep are being killed for the meat so maybe they are being primarily slaughtered to generate these lab products. Am I right for thinking this?
I’m asking these questions because I enjoy working in the lab but just want to know the cost associated with the products I use in regards to animal life. Also, can you think of any other animal-derived reagants/products that I left out that could be used in in-vitro experiments?
Thanks for any help you can provide.