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competent cells

competent cells - Protocols and Methods Forum

competent cells - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 06-20-2012, 08:38 PM
Pipette Filler
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Default competent cells



HI
do you experience storing competent cells at -20C degrees? Mine didn't survive :/ any reliable protocol for chemically competent cells?
txc
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Old 06-21-2012, 04:27 AM
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Default Re: competent cells

Hi Abigeil21,

I know I don't keep competent cells @ -20C. It should be -80C at least for longer storage. But be warn that competent cells don't usually remain competent (1 month competency is still good but less when time goes by.). Best to prepare FRESH.

If you are afraid your method maybe not optimized maybe you can share with us your protocol.
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