do you experience storing competent cells at -20C degrees? Mine didn't survive :/ any reliable protocol for chemically competent cells?
Re: competent cells
I know I don't keep competent cells @ -20C. It should be -80C at least for longer storage. But be warn that competent cells don't usually remain competent (1 month competency is still good but less when time goes by.). Best to prepare FRESH.
If you are afraid your method maybe not optimized maybe you can share with us your protocol.
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