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cloning with pGLA4.28 vector

cloning with pGLA4.28 vector - Protocols and Methods Forum

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Old 04-16-2012, 02:38 PM
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Default cloning with pGLA4.28 vector



hi all! im new to cloning and such and was hoping someone could help me. my supervisor has gvien me a construct (pGLA4.28) and told me to transform it into some competent cells. I used TOP10 by invitrogen, got some colonies, then purified using miniprep. however im a bit stuck as to what to do next, a digest or pcr sequence? the ultimate aim is stable transfection, so the construct included a hygromycin resistance gene, however im unsure to how select the correct clones after the miniprep step, as have both vector+gene and empty vector control? apologies for sounding ignorant on this one, but cant seem to find anything online
thanks
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