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-   -   cloning with pGLA4.28 vector (http://www.molecularstation.com/forum/protocols-methods-forum/85145-cloning-pgla4-28-vector.html)

drspoon 04-16-2012 02:38 PM

cloning with pGLA4.28 vector
 
hi all! im new to cloning and such and was hoping someone could help me. my supervisor has gvien me a construct (pGLA4.28) and told me to transform it into some competent cells. I used TOP10 by invitrogen, got some colonies, then purified using miniprep. however im a bit stuck as to what to do next, a digest or pcr sequence? the ultimate aim is stable transfection, so the construct included a hygromycin resistance gene, however im unsure to how select the correct clones after the miniprep step, as have both vector+gene and empty vector control? apologies for sounding ignorant on this one, but cant seem to find anything online
thanks


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