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Eukaryotic cell isolation

Eukaryotic cell isolation - Protocols and Methods Forum

Eukaryotic cell isolation - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 03-13-2012, 03:10 PM
Pipette Filler
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Default Eukaryotic cell isolation



Hallo all,

I am looking for a good protocol to do a eukaryotic cell isolation from a katydids.

I have never done this type of work and wonder if there are protocols out there, I allready did some literature search, but I am wondering if there arent any good textbooks out there.

The thing is, I can see the eukarryotic cells, but how do I start?
I was thinking to simply dissect some katydids and put the cells in some general cell medium with antibiotics?
But how should I collect the cells? Just by picking them? or do I need to centrifuge it?

thanks in advance
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