I am looking for a good protocol to do a eukaryotic cell isolation from a katydids.
I have never done this type of work and wonder if there are protocols out there, I allready did some literature search, but I am wondering if there arent any good textbooks out there.
The thing is, I can see the eukarryotic cells, but how do I start?
I was thinking to simply dissect some katydids and put the cells in some general cell medium with antibiotics?
But how should I collect the cells? Just by picking them? or do I need to centrifuge it?
thanks in advance