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Phage Display Problems

Phage Display Problems - Protocols and Methods Forum

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Old 10-20-2011, 02:43 PM
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Default Phage Display Problems



Hi,

I am working with Phage Display libraries and I am having issues amplifying. I bind to my target, elute, and titer with no problems. However, when I amplify and retiter, I do not see any plaques. I am using PEG 8000 to precipitate and I am just not seeing any pellets. I called technical support and they said that my LB should be at 5 g/L and the one I have been using is 10 g/L. Does anyone know if this makes too much of a difference or have any advice as to why I am having so much trouble amplifying? Thanks in advance!
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