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Any idea what ladder this is?

Any idea what ladder this is? - Protocols and Methods Forum

Any idea what ladder this is? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 10-01-2011, 07:47 PM
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Default Any idea what ladder this is?



Hi All,

I used some DNA reference ladder in a gel a few months back and I don't know where it came from or where it's gone. The whole lab has since been emptied and rearranged (as a result of fire/water damage) so I can't find any trace of the tube any more.Yes, I know I should have written the damn thing down when I used it.. But does anyone know the manufacturer? I thought it might have been Kapa biosystems but it's actually a little different. And I don't think it's Life either.

This is a 1.2% agarose gel run for about 25 minutes.

Anyway, any help would be appreciated.

Thanks!
Nat
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Old 10-02-2011, 02:52 AM
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Default Re: Any idea what ladder this is?

for a gel ran for 25 minutes, it is sure resolved very nice. I guess you must have use a different buffer.

I am sorry, I am not familiar with this ladder profile. But generall would be easy if you know what ladder is it then you can just google like 10kb ladder and check google image. Maybe you can start there.
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Old 10-04-2011, 08:24 AM
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Default Re: Any idea what ladder this is?




Its very hard to know which ladder it could be there are hundreds of types and many companies making them. However you can narrow things down - are there any masters or phd theses around? These are detailed and contain all products used.
What about papers/publications from the lab - these should have markers listed.

Also a good thing is usually the 10kb markers are quite similar - so if you have a known band you should be able to figure out others or if its the right one

To me it seems its a 10kb ladder as Butters mentioned. The bottom gel last lane is either degraded dna/plasmid or primer dimers and the other lanes seem to be either plasmid or genomic DNA.

Judging by the gel percent, its not genomic and its more like 10kb ladder and plasmid but finding out what you ran in your lanes, and looking at the publications/theses I think you will find out

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Old 10-04-2011, 10:18 AM
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Default Re: Any idea what ladder this is?

well, I was bore so I "google" around and what do I found...
[Only registered users see links. ]
seem possible.
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Old 10-05-2011, 07:37 AM
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Default Re: Any idea what ladder this is?

Hey guys and girls,

Thanks very much for your help, I wasn't able to see it in any theses but we only really have stuff thats's a year old or more. Gaduate studies likes to hold onto submitted theses for about a year before giving them back!

I had a look on that Fermentas site (never heard of 'em!) and it looks very much like their 10kb ladder. Thanks so much for all the help, really appreciate it!

Nat
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Old 10-05-2011, 01:10 PM
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Default Re: Any idea what ladder this is?

btw what is the buffer used and voltage apply? I am curious.
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Old 10-06-2011, 09:40 AM
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Default Re: Any idea what ladder this is?

The buffer was TE0.1 (Although I'll have to check that, may have been something else similar). Gel was a 1.2 agarose, Et Br stained (about 1.5uL). 125amps as far as I remember.
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Old 10-06-2011, 10:05 AM
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Default Re: Any idea what ladder this is?

Thanks!
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