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Unconsistent GFP expression

Unconsistent GFP expression - Protocols and Methods Forum

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Old 09-27-2011, 11:09 AM
Pipette Filler
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Default Unconsistent GFP expression



Hi all,
I'm tryint to study the activity of my promoter by fusing it to the reporter gene GFP. The organism that I use is Pseudomonas aeruginosa.
For unknown reasons that scape to my understanding I'm not able to get consistent results despite that I always do the same protocol and that I control all conditions that I'm concernt.
Can someone help me?
Thanks in advance!
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