Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


How to reduce the specific activity of a radioactive compound?

How to reduce the specific activity of a radioactive compound? - Protocols and Methods Forum

How to reduce the specific activity of a radioactive compound? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 08-16-2011, 12:39 PM
Pipette Filler
Points: 4, Level: 1 Points: 4, Level: 1 Points: 4, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default How to reduce the specific activity of a radioactive compound?



Hi,

I was wondering if anyone could shed some light on how to reduce the specific activity of a compound.

I have a tritiated compound required for my experiment but I worked out at the concentrations I need I would be using a vial for every replicate!

So I need to reduce the specific activity but I am not sure how to do this. Presumably you do this with unlabeled compound? I have some unlabeled compound but its in a powder, I assume I add this to the radioactive compound but won't it affect the concentration?

Also, will you need extra time to count each sample? I currently count at 1 min per sample.

Thanks for the clarification!!
Reply With Quote
Reply

Tags
activity , compound , radioactive , reduce , specific


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Help- aCGH Agilent Arrays Helena Suarez Microarrays Forum 1 03-17-2011 06:24 PM
how to reduce IgG non specific binding? dizue Antibody Forum 2 12-02-2008 09:42 AM
Help with calculations for specific activity Fasulo, Lisa M Protein Forum 4 10-15-2008 11:07 PM
Calculations for specific activity Morales, Victor Protein Forum 0 10-02-2008 01:17 PM
how to incorporate radioactive sulphur in specific proteins irfan_sadiq Recombinant Protein Forum 0 01-26-2008 10:32 AM


All times are GMT. The time now is 07:29 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11126 seconds with 16 queries