Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


what is the optimal wavelength used to read luminescence in a plate reader?

what is the optimal wavelength used to read luminescence in a plate reader? - Protocols and Methods Forum

what is the optimal wavelength used to read luminescence in a plate reader? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 04-10-2011, 07:44 PM
Pipette Filler
Points: 4, Level: 1 Points: 4, Level: 1 Points: 4, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default what is the optimal wavelength used to read luminescence in a plate reader?



I'm using an assay kit to read proteosomal activity in cancer cells. This assay is performed in a 96-well plate reader, and data is acquired by measuring luminescence.

I noticed that, unlike fluorescence measurements, where a maximun emission wavelength to read your sample in a plate reader is often specified by the manufacturer instructions, for luminescence readings, I don't often see a specified wavelength. At least I have not with this kit I'm using and I'm not sure why that is. Can luminescence be read at different wavelengths?

At least with the instrument we're using, the option that gave the best signal was an ''all nm'' option, instead of choosing a specific wavelength when making the measurement. I wonder if these readings are reliable. Any information is highly appreciated!
Reply With Quote
  #2  
Old 01-18-2012, 12:15 PM
Pipette Filler
Points: 27, Level: 1 Points: 27, Level: 1 Points: 27, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2012
Posts: 8
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: what is the optimal wavelength used to read luminescence in a plate reader?

Luminescence and fluorescence are simply different methods of measurement, don't try to understand one with the basics of the other!
In luminescence you don't need to excite a fluorophore, the sample is simply producing light (gamma rays) and you quantify the amount of light. The wavelength is irrelevant here, nor do you need any filter.
Reply With Quote
Reply

Tags
luminescence , optimal , plate , read , reader , wavelength


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
The Theory of Moving Dimensions: The Time Dimension is Moving Relative to The Three Spatial Dimensions Captain Ranger McCoy Physics Forum 14 07-03-2005 03:14 AM


All times are GMT. The time now is 03:19 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11641 seconds with 16 queries