I'm using an assay kit to read proteosomal activity in cancer cells. This assay is performed in a 96-well plate reader, and data is acquired by measuring luminescence.
I noticed that, unlike fluorescence measurements, where a maximun emission wavelength to read your sample in a plate reader is often specified by the manufacturer instructions, for luminescence readings, I don't often see a specified wavelength. At least I have not with this kit I'm using and I'm not sure why that is. Can luminescence be read at different wavelengths?
At least with the instrument we're using, the option that gave the best signal was an ''all nm'' option, instead of choosing a specific wavelength when making the measurement. I wonder if these readings are reliable. Any information is highly appreciated!