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Extracting Microflora from Skin Surface

Extracting Microflora from Skin Surface - Protocols and Methods Forum

Extracting Microflora from Skin Surface - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 04-06-2011, 12:06 PM
Els Els is offline
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Default Extracting Microflora from Skin Surface



Hi -

I am looking for methods to reliably extract skin microflora samples from the skin surface, including skin folds, sweat glands, and hair follicles. The technique will acquire flora from down hair follicles, sweat glands & other skin structures without harming the underlying skin substrate. Technique needs to be robust, consistently acquiring at least 20% of the microbes present in the various testing areas and must deliver a representative sampling of microbes present with repeatable confidence & facilitate the extraction of DNA/RNA from the sample.

It must not destroy the skin surface or treat it harshly. Sampling will be at a rate of about 100/week, with an average of 2ml per sample.

Safe, non-toxic, non-irritant to people being sampled, non-destructive to skin. Must not contaminate subsequent procedures. May be destructive to the microbe but not the DNA/RNA.

Any suggestions?

Thanks!
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dna , extracting , microflora , rna , sampling , skin , surface


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