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293 cell culture at low temperature
I am trying to do a temperature block at 20 C to follow an expressed protein along the secretory pathway. This has been reported using other cell types, ie. Hela, etc.
I am using 293 T cells. However, they don't seem to be very happy at low temperature incubation. Has anybody attempted this? Have you been succesful? Any tips greatly appreciated.
Re: 293 cell culture at low temperature
20C is pretty low, most secretory experiments I know of work in the range of 30C.
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