| | Re: Biology newbie in need of help regarding autoclaving
generally in molecular biology labs we autoclave most things that can be autoclaved - this includes pipette tips, equipment such as glassware, and even buffers and water in glassware.
The reason being if you spend X amount of time and money, you do not want to lose them both because you didn't want to spend a bit of time autoclaving. Autoclaving only costs time, not money usually. Even better, get a bunch of tips, glassware and buffers all at once - autoclave them - and they can last you your whole project probably.
Some people cut corners, but you have to assess where you can cut them. I would mainly keep on the safe side and work with the peptide sterile (using autoclaved stuff), because the last thing you want to find is that you have contamination in your stocks - or your amazing findings are actually due to contamination - or worse that they your findings may be lost or be complicated by things growing in them.
I think it is important to also find out if autoclaving will affect your experiments negatively, does it generate nanoparticles that may compromise your study? I am not sure about this but would pay to look it up - what do others in your field do?
Last edited by admin; 02-22-2011 at 03:59 PM.