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RNA isolation from Trichoderma

RNA isolation from Trichoderma - Protocols and Methods Forum

RNA isolation from Trichoderma - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 01-11-2011, 08:42 AM
Pipette Filler
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Default RNA isolation from Trichoderma



I have tried trizol method and qiagen plant mini kit for isolation of RNA from Trichoderma but I end up with no RNA. So anyone working on Trichoderma or any filamentous fungi please sort me out of this.
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Old 02-24-2011, 07:07 AM
Pipette Filler
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Default Re: RNA isolation from Trichoderma

It could be something as basic as it's getting degraded. Are you using everything that's RNAse free? DEPC water? Sterile filter tips?
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