Ok, I have seen that a lot of people have trouble with understanding this dilutions and concentration calculations.. and I would like to adress someone who has a lot of patience to explain to me this. I did Bradford the first time, so i had wide range of standards.. and i dont know how to calculate the concentration of standards!!! so if you explain me how to calculate this, i will know it once and for all,. i have my own theory but lets keep it a secret for now. so, to resolve this very "complicated issue", i will tell you that for the first standard i added
4 ul od BSA stock (1 mg/ml),
196 ul of buffer (PBS),
and 50 ul of Bradford reagent..
so i guess the TOTAL Volume is 250 ul? i dont even know that for sure, does the reagent volume counts also?
so that's pretty much it, and.. what is my concentration?