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Transfection method for MEF cell line

Transfection method for MEF cell line - Protocols and Methods Forum

Transfection method for MEF cell line - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 11-18-2010, 10:35 AM
Pipette Filler
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Default Transfection method for MEF cell line



Hello,

i need help to accomplish transfection to MEF cell line. So far, i tried turbofect reagent with different cell numbers and different amounts of EGFP plasmid (since im checking the transfection efficiency under flourescent microscop), but they were not successful, however in fermentas web page it is written i can be.. (according to costumer feedback)

is there anybody who worked with MEF cells and accomplished transfection with them? if so, i would like to learn what were the details??

thnx
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