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#1
07-14-2010, 03:02 PM
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 cloning DH5a cells translation Hi, Does anyone know a way to slowdown/ inhibit translation in e.coli? I want to clone a gene in DH5alpha cells, but it seems to get expressed and it thereby kills my cells. hope someone can help me out! Thanks, VJ   |
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#2
07-14-2010, 04:50 PM
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| Re: cloning DH5a cells translation Clone it into a vector with an inducable promotor - i.e. IPTG on. That way you can clone with the vector in an "off position", and then turn it on when you want the protein produced. |
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| cells , cloning , dh5a , dh5alpha , e.coli , gene expression , translation |
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