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cloning DH5a cells translation

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Old 07-14-2010, 03:02 PM
Pipette Filler
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Default cloning DH5a cells translation



Hi,
Does anyone know a way to slowdown/ inhibit translation
in e.coli? I want to clone a gene in DH5alpha cells, but it seems to get expressed and it thereby kills my cells.
hope someone can help me out!
Thanks,
VJ
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Old 07-14-2010, 04:50 PM
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Default Re: cloning DH5a cells translation

Clone it into a vector with an inducable promotor - i.e. IPTG on. That way you can clone with the vector in an "off position", and then turn it on when you want the protein produced.
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cells , cloning , dh5a , dh5alpha , e.coli , gene expression , translation


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