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How many hours do B cells need to turn into plasma cells?

How many hours do B cells need to turn into plasma cells? - Protocols and Methods Forum

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Old 06-14-2010, 09:11 PM
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Default How many hours do B cells need to turn into plasma cells?



Hello.
I would like to know how many hours (after anti-CD3 stimulation in vitro) B cells need to change into plasma cells.

Last edited by CD19; 06-14-2010 at 09:41 PM.
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Old 09-07-2012, 01:18 PM
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Default Re: How many hours do B cells need to turn into plasma cells?

I see noone replied. I will tell you in few months.
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Old 09-10-2012, 10:58 AM
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Default Re: How many hours do B cells need to turn into plasma cells?

Anti-CD3 soluble or coated on polystyrene plate (dispense 50 µl of 5-10 μg/ml anti-CD3 [OKT3 – Ortho] in sterile PBS incubating for 24 hrs at 4 °C) react with surface membrane T cell receptor (peripheral blood mononuclear suspension in RPMI 1640 medium with 5 % FCS) inducing, after ~16 hrs at 37 °C in 5 % CO2 incubation, cytokines production to achieve stimulus, IL-2 play a major role, on naive B cell changing in B cell blast after ~ further 8 h. From here, time of plasma cell development depending from B-cell subset phenotype (MZ/B1 and B2) with generate plasma blast after respectively 6 and 48 h further. For develop mature plasma cell, is need one progressive down-regulated with rearrangement that to lead at loss of many surface cluster differentiation antigens what it ends in ~24 hrs. The computation time for to complete entire process is ~96 hrs (4 days).
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Old 10-19-2012, 08:10 PM
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Default Re: How many hours do B cells need to turn into plasma cells?

Where did you take it from?
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Old 10-21-2012, 03:57 PM
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Default Re: How many hours do B cells need to turn into plasma cells?

For plasma cell developing time
- Encyclopedia of Life Sciences - Heather A Minges Wols - Plasma cell - Ed. 2005
- Kirsten A. Fairfax, Axel Kallies, Stephen L. Nutt, David M. Tarlinton (2008) Review : Plasma cell development: From B-cell subsets to long-term survival niches. Seminars in Immunology 20, 49–58
- Miriam Shapiro-Shelef and Kathryn Calame (2005) Review : Regulation of plasma-cell development. Nature 5, 230-242.
For anti-CD3 coated plate for B cell stimulation in mixed PBMC culture, data arising by my experience partly presented in one my article "F. Zagami (1994/1995) Proliferative response of the lymphocytes to mitogens. G. It. Patol. Clin. 1-4, 28-34 (Italian)", and
partly data never published, both free available on request.
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