| | Re: Affinity purification with organic solvents at pH 7.5
Thanks for replying.....Ya I went through many company catalogs whoever supply the resin, but without success on information about the organic solvents. I assume that it is not compatible as acetonitrile has pH of ~1-2. But then my idea was to mix acetonitrile with 50% tris buffer so that pH can be adjusted to around 7.5-8. Just working conditions with the buffers what would happen by mixing them with and without protein.
As you mentioned, if Nickel is a catalyst in hydrogenation of of nitrile molecule, what do you think can happen ????
I understood that it doesn't change the Nickel ion bound to resin in any form (oxidation/reduction) by acting as a catalyst. And if acetonitrile is hydrogenated, I don't think, protein can be damaged. If you have more information, please let me know.