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Homogenization for producing pure plasma membrane free ER-microsomes

Homogenization for producing pure plasma membrane free ER-microsomes - Protocols and Methods Forum

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Old 02-10-2010, 01:58 PM
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Default Homogenization for producing pure plasma membrane free ER-microsomes



I'm working with algal cells and would like to isolate ER from the rest of the cell's components for phospholipid make-up characterization. However I'm concerned with the homogenization step as most methods give microsomes that have both ER-membrane and plasma membrane patches. Has anyone got ideas for getting pure ER-membrane microsomes?
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