Hi guys, this is my very first post, so if this is the wrong section, please move/ remove!
I've been searching for a quantitative assay that will allow me to quantify the number of eukaryotic cells in a gel along with bacteria infected. The eukaryotic cells are going to be fibroblasts and bacteria will be e. coli, pseudomonas aeruginosa, and staph aureus.
I'm hoping I won't have to do staining/ sectioning if I can avoid it. So something similar to an MTT assay, an enzymatic assay that works on eukaryotic cells and not on prokaryotic cells would be fantastic!
Any suggestions would be greatly appreciated!