I would like to conduct a simple viability count for yeast in the brewery using methylene blue (MB) stain...the protocol states that I dilute my 0.1% stock MB solution tenfold with "0.1M Glycine Buffer (pH 10.6)" . Can one of you pros please tell me the recipe for this buffer? The protocol also mentions that citric acid is required but fails to mention what it is to be used for...any ideas on that?
I apologize for not being a microbiologist and wasting your time if this is a dumb question...I only want to brew good beer and someone here can surely help.
Thanks in advance for any links/help.
"w w w.whitelabs.c o m/beer/alkaline.h t m l" is a link to the protocol (no spaces)