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Simple question on stains and buffers

Simple question on stains and buffers - Protocols and Methods Forum

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  #1  
Old 01-19-2010, 09:26 PM
Pipette Filler
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Default Simple question on stains and buffers



I would like to conduct a simple viability count for yeast in the brewery using methylene blue (MB) stain...the protocol states that I dilute my 0.1% stock MB solution tenfold with "0.1M Glycine Buffer (pH 10.6)" . Can one of you pros please tell me the recipe for this buffer? The protocol also mentions that citric acid is required but fails to mention what it is to be used for...any ideas on that?

I apologize for not being a microbiologist and wasting your time if this is a dumb question...I only want to brew good beer and someone here can surely help.

Thanks in advance for any links/help.

"w w w.whitelabs.c o m/beer/alkaline.h t m l" is a link to the protocol (no spaces)
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  #2  
Old 01-20-2010, 10:25 AM
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Default Re: Simple question on stains and buffers

citric acid was actually for citrate methylene blue solution as in link below, i guess the author must have mistake it. You can contact the webmaster for clarification
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referring to link below, you use

7.5g of glycine,
add water till 1L
*and adjust pH to 10
or
0.75g of glycine
add water till 100mL
*adjust pH to 10

*i usually adjust the pH first before adding water to the desire final volume

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if i am wrong anywhere please correct me
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  #3  
Old 01-21-2010, 04:32 PM
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Default Re: Simple question on stains and buffers

Ah...I see. Thank you very much and cheers!
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Old 06-01-2012, 05:03 AM
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Default Re: Simple question on stains and buffers

thanks for the clarification
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