|Register||Search||Today's Posts||Mark Forums Read|
|Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.|
| ||LinkBack||Thread Tools||Display Modes|
Hanging Drop Aggregation Assay
I was reading some papers in which this method was employed but I don't understand one part of the procedure. Maybe this is because I'm missing something behind the basis of the method. Can someone explain me?
My doubt is: for quantification, some protocols say "Resulting cell clusters were subjected to 30 rounds of pipetting through a 200-ul Gilson pipette, and the degree of dissociation was quantified by counting the particles after trituration". I don't get this part...
So, after the incubation the drops/clusters of cells are still in the lid of the plate, right? What am I supposed to do with them?
And another question: how many drops per well should I put in the lid of a 24-well plate?
|aggregation , assay , drop , hanging|
|Thread||Thread Starter||Forum||Replies||Last Post|
|Bradford protein assay, thanks!||huzazhang||Protein Science||1||05-25-2010 08:39 AM|
|Question on MTT assay||mcdeena||Molecular Biology Techniques||0||04-06-2010 03:32 PM|
|help with hanging drop cryst.||karenneedham||Molecular Biology Techniques||0||10-27-2008 09:19 PM|
|CAM assay and Transformation assay||Alvin Acebedo||Protocols and Methods Forum||0||05-28-2006 02:18 AM|
|Biorad Bradford Assay||Deanne Bell||Protocols and Methods Forum||0||05-13-2004 03:21 PM|