I'm doing a PhD in horse virology and it is more than 4 months that I can not develop an efficient protocol for Immunofluorescence of infected PBMC cells
I am using a monoclonal antibody that binds to a nucleocapsid protein. This product is available on the market
my problem is that this mAb binds aspecifically neutrophils (you can identify them for the morphology of the nucleus ... the really strange thing is that using an irrelevant isotype autofluorescence does not develop!
I tried to use a protocol to limit autofluorescence but was not successful
I do not know what to do, time passes and I always stop at the same point
I hope you can help