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(no subject) - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 11-03-2009, 04:58 PM
Allan Jones
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Hi!

Does anyone here know how stable Adenovirus (not replcation competent) is in mice? I was weighing out pulverized liver tissue of such mice and when cleaning my spatula afterwards with a wet cloth, splashed some of the liquid into my face (incl. lips). My labmates said that there will be very little to no intact virus in the animals after seven days,when the experiment ende (as it is not replication competent and should have either infected cells or been cleared by the mouse's immune system after this time).

Does anyone here know if this isthecase, as i am a little worried about having an AV expressing shRNA in my cells. The doc in the building noted the accident, saying that replication incompetent viruses will not so any harm, especially Adenovirus, as it does not integrate, but i am still a little worried and unsure of how to handle this.

Best Wishes!
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Old 11-05-2009, 02:58 PM
StewJW
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Shouldn't you have been doing this work in something like a class 2
cabinet? Anyhow to quote from a recent paper:

Endogenous retroviruses (ERVs) represent the proviral phase of
exogenous retroviruses that have integrated into the germ line of
their host . They typically consist of an internal region with three
genes (gag, pol, and env) plus two flanking, noncoding LTRs, which are
identical at the time of integration. Human ERVs (HERVs) comprise ≈5–
8% of the human genome.

As long as it doesn't integrate into anywhere critical it'll just be
one more on the list. Anyhow if its not replication competent surely
that answers your question. I should add I know very little about this
area but I did find your dilemma quite amusing.
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Old 11-05-2009, 11:58 PM
mjr
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On 2009-11-03, Allan Jones <[Only registered users see links. ]> wrote:
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