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clean-up of ssDNA

clean-up of ssDNA - Protocols and Methods Forum

clean-up of ssDNA - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 09-22-2009, 03:58 PM
Priyesh Rughani
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Default clean-up of ssDNA



Dear Tom,

A while ago you posted a thread (see below), I was wondering if you could share the link to the protocol you used for the generate ssDNA.
I have searched the web, but cant not find the protocol you mentioned

Thanks,
Priyesh Rughani




Dear Colleagues,



I have isolated mg quantities of ssDNA following the protocol of Gruber et

al in Focus 15: 59-65. I have a problem in that the OD 260/280 is about 1.5

after following the full protocol. I need to quantify this material precisely

as well as having very clean ssDNA.

Does anyone have any quick solutions for a cleanup of this large amount of

material. In the protocol, multiple phenol-chloroform extractions have

already been performed.

If there is a FAQ to this point could someone direct me to it.



Thanks,



Tom Newman

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