A while ago you posted a thread (see below), I was wondering if you could share the link to the protocol you used for the generate ssDNA.
I have searched the web, but cant not find the protocol you mentioned
I have isolated mg quantities of ssDNA following the protocol of Gruber et
al in Focus 15: 59-65. I have a problem in that the OD 260/280 is about 1.5
after following the full protocol. I need to quantify this material precisely
as well as having very clean ssDNA.
Does anyone have any quick solutions for a cleanup of this large amount of
material. In the protocol, multiple phenol-chloroform extractions have
already been performed.
If there is a FAQ to this point could someone direct me to it.