|Register||Search||Today's Posts||Mark Forums Read|
|Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.|
| ||LinkBack||Thread Tools||Display Modes|
clean-up of ssDNA
A while ago you posted a thread (see below), I was wondering if you could share the link to the protocol you used for the generate ssDNA.
I have searched the web, but cant not find the protocol you mentioned
I have isolated mg quantities of ssDNA following the protocol of Gruber et
al in Focus 15: 59-65. I have a problem in that the OD 260/280 is about 1.5
after following the full protocol. I need to quantify this material precisely
as well as having very clean ssDNA.
Does anyone have any quick solutions for a cleanup of this large amount of
material. In the protocol, multiple phenol-chloroform extractions have
already been performed.
If there is a FAQ to this point could someone direct me to it.
|cleanup , ssdna|
|Thread||Thread Starter||Forum||Replies||Last Post|
|running ssDNA (30-1000nt) in denatured conditions||TC||Protocols and Methods Forum||1||11-19-2008 07:10 AM|
|Western Blot Woes - Very Clean Western||kiki06||Western Blot Forum||3||07-22-2008 02:30 PM|
|Poor resolution when isolating ssDNA?||Evan Reed||Protocols and Methods Forum||1||06-17-2008 06:12 AM|
|'clean, fresh scent'?||louis||Chemistry Forum||1||02-21-2004 07:50 PM|
|FS: Old Physics-Related Books||Oldbooks78||Physics Forum||0||08-27-2003 02:32 PM|