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Methods Digest, Vol 52, Issue 7

Methods Digest, Vol 52, Issue 7 - Protocols and Methods Forum

Methods Digest, Vol 52, Issue 7 - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 09-12-2009, 03:23 AM
Virash Gupta
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Default Methods Digest, Vol 52, Issue 7



Besides all other answers, try this- If plasmid is there it will be
isolated. If you using old solutions or old kit for plasmid purification-
the main culprit is solution 2- alkaline lysis solution. It contains NaOH
and as it gets repeatedel exposed to air-it absorbs carbon dioxide and
converted to sod carbonate- to result in decreased alkalinity. The
solution becomes inefficient in appropriate lysis. So prepare fresh solution
and use.

On 9/11/09, [Only registered users see links. ] <
[Only registered users see links. ]> wrote:



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Dr V K Gupta
Sr Microbiologist (Molecular Biology)
Insect Molecular Biology Lab
Department of Entomology
Punjab Agricultural University
Ludhiana (Pb)-141004- India
M: 09815963210
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