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#1
09-12-2009, 03:23 AM
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 Methods Digest, Vol 52, Issue 7 Besides all other answers, try this- If plasmid is there it will be isolated. If you using old solutions or old kit for plasmid purification- the main culprit is solution 2- alkaline lysis solution. It contains NaOH and as it gets repeatedel exposed to air-it absorbs carbon dioxide and converted to sod carbonate- to result in decreased alkalinity. The solution becomes inefficient in appropriate lysis. So prepare fresh solution and use. On 9/11/09, [Only registered users see links. ] < [Only registered users see links. ]> wrote: -- Dr V K Gupta Sr Microbiologist (Molecular Biology) Insect Molecular Biology Lab Department of Entomology Punjab Agricultural University Ludhiana (Pb)-141004- India M: 09815963210  |
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