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Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


pfu pc

pfu pc - Protocols and Methods Forum

pfu pc - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 08-11-2009, 09:47 PM
Jon Burdach
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Default pfu pc



Try adding 10% DMSO to your PCR tube. It helps prevent secondary structure
formation for G-C rich amplicons.

I was trying to get a pfu PCR working for ages and it worked perfectly as
soon as I added DMSO.

Jon
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