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Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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pfu pcr - Protocols and Methods Forum

pfu pcr - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 08-10-2009, 05:33 PM
David-Paul Minde
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.... if your amplicon is "big" (e.g. 5-15 kb), you better choose phusion
polymerase (or the like) and it will just work ...(unless your template or
primers are degraded or you have problems with primer sequence specificity
which can be overcome by prolongation in many cases)
best wishes,
David

2009/8/10 malihe keramati <mlh_keramati@yahoo.com>




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