pfu pcr

.... if your amplicon is "big" (e.g. 5-15 kb), you better choose phusion
polymerase (or the like) and it will just work ...(unless your template or
primers are degraded or you have problems with primer sequence specificity
which can be overcome by prolongation in many cases)
best wishes,
David

2009/8/10 malihe keramati <mlh_keramati@yahoo.com>




--
David Minde MSc (TUM)

Cellular Protein Chemistry Room 707
Department of Chemistry
Faculty of Science
Utrecht University
Krytgebouw
Padualaan 8
NL-3584 CH Utrecht
The Netherlands

office phone +31 30 253 4105
(magic android +31 652614443

home:
Griftkade 4 bis
3572 TW Utrecht

Science is what happens while we are making other plans (~John Lennon)