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storing of enzyme

storing of enzyme - Protocols and Methods Forum

storing of enzyme - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 07-28-2009, 07:51 PM
Dr. Hiranya S. Roychowdhury
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Default storing of enzyme



Use a buffer (10mM Tris-EDTA, pH 6.5 to 7.2, or 50mM phosphate buffer
should work) and add glycerol to 35% (as high as 50%). Store in a -20C
freezer (no auto defrost).

Cellulase can also be stores freeze dried after the appropriate dialysis
against distilled water containing 1mM DTT.



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Hiranya S. Roychowdhury, Ph.D.
Asst. Professor,
Health & Public Services
Dona Ana Community College
New Mexico State University
Las Cruces, NM 88003

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  #2  
Old 07-28-2009, 09:55 PM
Nick Theodorakis
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Default storing of enzyme

On Jul 28, 3:51*pm, "Dr. Hiranya S. Roychowdhury" <[Only registered users see links. ]>
wrote:

Is dialysis tubing still made from cellulose?

Nick

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  #3  
Old 07-29-2009, 12:56 AM
DK
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Default storing of enzyme

In article <[Only registered users see links. ]>, Nick Theodorakis <[Only registered users see links. ]> wrote:

Good point! Yes, they are. With cellulase, it might be a problem :-)
Fortunately, there is an alternative: membranes made from
cellulose acetate (what Spectrum calls "Cellulose Ester"). Those
would be extemely unlikely to be hydrolysed by cellulase.

DK
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  #4  
Old 07-29-2009, 03:26 PM
Dr. Hiranya S. Roychowdhury
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Default storing of enzyme

Mostly cellulose nitrate. I had done it "way back when ..." using these.




--
Hiranya S. Roychowdhury, Ph.D.
Asst. Professor,
Health & Public Services
Dona Ana Community College
New Mexico State University
Las Cruces, NM 88003

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