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Endotoxins

Endotoxins - Protocols and Methods Forum

Endotoxins - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 07-27-2009, 10:23 AM
Yvonne Couch
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Default Endotoxins



Hi all,

If I HCl purify a peptide made in E.Coli, is there likely to be a large
amount of endotoxic material left in the final solution and if so is it
possible to get rid of this?

Cheers

Yvonne

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  #2  
Old 08-04-2009, 08:43 AM
StewJW
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Default Endotoxins

You could use a 10,000 MWCO ultrafiltration filter. There are a number
of manufacturers who produce these depending on the solution volume. A
LAL test will determine the endotoxins levels.

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  #3  
Old 08-04-2009, 09:00 AM
StewJW
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Default Endotoxins

Just add typical filter manufacturers who supply ultrafilters are
Sartorius, PAL, Millipore, Whatman etc. Another method I know little
about is polymyxin B immobilized on Sepharose 4B; I just noticed GE
Life Science have removed this from their catalogue, maybe not a
popular method. I also came across the review from a Google search,
sadly my main source of info these days:

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