Endotoxins

Hi all,

If I HCl purify a peptide made in E.Coli, is there likely to be a large
amount of endotoxic material left in the final solution and if so is it
possible to get rid of this?

Cheers

Yvonne

You could use a 10,000 MWCO ultrafiltration filter. There are a number
of manufacturers who produce these depending on the solution volume. A
LAL test will determine the endotoxins levels.

Just add typical filter manufacturers who supply ultrafilters are
Sartorius, PAL, Millipore, Whatman etc. Another method I know little
about is polymyxin B immobilized on Sepharose 4B; I just noticed GE
Life Science have removed this from their catalogue, maybe not a
popular method. I also came across the review from a Google search,
sadly my main source of info these days:

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