qPCR NEWS July 2009 - focus on single-cell qPCR

qPCR NEWS July 2009 - focus on single-cell qPCR
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Dear researcher,
dear Gene Quantification page reader,

Our newsletter informs about the latest news in quantitative real-time
PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene
Quantification homepage. The focus of this newsletter issue is:

- update in single-cell real-time PCR
- qPCR / real-time PCR BLOG => [Only registered users see links. ]
- qPCR LinkedIN group - real-time PCR & qPCR =>
[Only registered users see links. ]
- For better navigation we developed a TAG CLOUD =>
[Only registered users see links. ]
- New qPCR events in autumn 2009: symposia & workshops =>
[Only registered users see links. ]

European wide qPCR application workshops => register now !
=> course program spring - summer - autumn 2009
=> [Only registered users see links. ]
=> [Only registered users see links. ]


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Single-cell molecular-biology is a relatively new scientific branch in
biology. The first single-cell analysis were involved in the
characterization of mitochondrial DNA in 1988. Single-cell DNA
analysis, in particular genomic DNA, is important and may be
informative in the analysis of genetics of cell clonality, genetic
anticipation and single-cell DNA polymorphisms. Nowadays for most
scientists the quantitative transcriptomics in a single-cell is much
more important, and the analytical method of choice is the
quantitative real-time RT-PCR. The relative abundance of single mRNAs
and their up- or down-regulation in a single cell, compared to their
neighbour cells, is the goal. The need for quantitative single-cell
mRNA analysis is evident given the vast cellular heterogeneity of all
tissue cells and the inability of conventional RNA methods, like
northern blotting, RNAse protection assay or classical block RT-PCR,
to distinguish individual cellular contributions to mRNA abundance
differences.

=> [Only registered users see links. ]


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new papers => [Only registered users see links. ]

- Imaging intracellular RNA distribution and dynamics in living
cells.
- mRNA-Seq whole-transcriptome analysis of a single cell.
- Genomic expression analysis by single-cell mRNA differential display
of quiescent CD8 T cells from tumour-infiltrating lymphocytes obtained
from in vivo liver tumours.
- Rac1 regulates pancreatic islet morphogenesis.
- A novel single-cell quantitative real-time RT-PCR method for
quantifying foot-and-mouth disease viral RNA.
- Quantification of circulating endothelial and progenitor cells:
comparison of quantitative PCR and four-channel flow cytometry.
- Prognosis of non-small cell lung cancer patients by detecting
circulating cancer cells in the peripheral blood with multiple marker
genes.
- Nanoliter reactors improve multiple displacement amplification of
genomes from single cells.
- Fluidigm Dynamic Arrays provide a platform for single-cell gene
expression analysis.
- Intracellular expression profiles measured by real-time PCR
tomography in the Xenopus laevis oocyte.
- Quantification of mRNA in single cells and modelling of RT-qPCR
induced noise.
- Intracellular Gene Expression Profi les Revealed with Real-time PCR
Tomography


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NEW blogs & groups

qPCR / real-time PCR BLOG => [Only registered users see links. ]

qPCR LinkedIN group - real-time PCR & qPCR =>
[Only registered users see links. ]

This group wants to join worldwide users in the field of real-time
quantitative PCR. It offers a forum where new ideas can be exchanged,
and allows networking with other people in this field.


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Upcoming Events World-wide academic and commercial qPCR Events
[Only registered users see links. ]

Symposia, Meetings, Conferences, Workshops, Seminars, Online-Seminars,
qPCR Education Program, etc.
Please submit your qPCR event here => events@gene-
quantification.info


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qPCR WORKSHOP

BioEPS GmbH / TATAA Biocenter Germany - qPCR Application workshops

At the TATAA Biocenter Germany we offer qPCR application workshops, a
3-day qPCR Core Module and a 2-day qPCR Biostatistics Module. All
courses are held regularly in Göteborg, Sweden, in English and in
Freising-Weihenstephan, Germany, in German and English, and in Prague,
Czech Republic in English and Czech.
Depending on the occasion the workshop language and the different
prices may apply. Further customized workshops and specialized
trainings will be held as well across Europe and world-wide.
TATAA Biocenter Germany workshops are held in cooperation with BioEPS
GmbH, located at the campus of the Technical University of Munich, in
Freising-Weihenstephan, very close to the Munich Airport (MUC). For
more information and registration, please see our web page:
=> [Only registered users see links. ]


Course Occasions 2009:

- 3-day qPCR Core Module (Mon. - Wed.)
- 2-day BioStatistics Module (Thu. - Fri.)
- 3-day single-cell qPCR Module (Mon. - Wed.)
- 3-day microRNA Module (Mon. - Wed.)
=> [Only registered users see links. ]

14 - 16 September 2009 (E) NEW single-cell qPCR
19 - 23 September 2009 (E)
3-day microRNA Module (Mon. - Wed.) & 2-day BioStatistics (Thu. -
Fri.)
26 - 28 October 2009 (E) 3-day qPCR Core Module (Mon. - Wed.)
16 - 20 November 2009 (E)
3-day microRNA Module (Mon. - Wed.) & 2-day BioStatistics (Thu. -
Fri.)
7 - 11 December 2009 (E)
3-day qPCR Core Module (Mon. - Wed.) & 2-day BioStatistics (Thu. -
Fri.)

=> [Only registered users see links. ]
=> [Only registered users see links. ]


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Forward Please send the qPCR NEWS to further scientists and friends
who are interested in qPCR !


Best regards,

Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages
[Only registered users see links. ]


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