|Register||Search||Today's Posts||Mark Forums Read|
|Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.|
| ||LinkBack||Thread Tools||Display Modes|
I am working with AFLP,s in elasmobrachs, and at the beginning I get pretty good result, however, after a few samples working well, now I can not get any peak in my chromatogram, I have check the quality of DNA, very good, I have check that the digestion/ligation working well, and even my preselect amplification produce a great smear between 100 and 1500 pb. But when I run final PCR product in an agarose gel, I get as bright smear in from 500 to the tank (the hole when you put the DNA), and of course I donít get nothing in the chromatogram.
I have changed concentration of MgCl and DNA, I have changed the number of cycles in the PCR profile, I have changed all my chemicals, and nothing has worked.
So, any idea of what could produce the smear at large number of pb?
|aflp , smear|
|Thread||Thread Starter||Forum||Replies||Last Post|
|pcr smear||bhaktamehul||PCR - Polymerase Chain Reaction Forum||27||01-15-2013 07:57 AM|
|AFLP low yield||aria johnson||Protocols and Methods Forum||1||06-03-2009 10:54 AM|
|Smear following Formamide/Formaldehyde denaturation of RNA||cmcthorne||RNA Techniques Forum||2||02-16-2009 08:21 AM|
|MSP product looks like some smear||Amily||Epigenetics Forum: DNA Methylation, Histone and Chromatin Study||2||10-16-2007 04:22 AM|
|FW: AFLP||Deanne Bell||Protocols and Methods Forum||0||05-11-2004 06:23 PM|