I am working with pET vector system. for expression of the enzme in
I have to design primer with Nco1 site in forward primer.
so can anybody will suggest me about the placing of ATG site of
ORF...whether it should be in the primer's R.E site or else again i
have to add in the primer aftre the R.E site.
Historians believe that in newspost
<[Only registered users see links. ].ne t> on Sun, 19 Jul 2009,
Dr. Hiranya S. Roychowdhury <[Only registered users see links. ]> penned the following
Just remember that for Nco I, the codon following the ATG must start
with a G either original or you must add one i.e. CC - ATG - GNN
Also pick the correct pET21 i.e. pET21d :-)
For this reason I much prefer to use Nde I whenever possible i.e.
CATATG. No problems with extra aa's.
I love deadlines. I especially like the whooshing noise they make as
they go flying by.